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. Author manuscript; available in PMC: 2016 Jun 28.
Published in final edited form as: Bio Protoc. 2016 Jun 5;6(11):e1833. doi: 10.21769/bioprotoc.1833

Figure 2. Atherosclerosis lesion analysis of whole aorta.

Figure 2

Removal of perivascular adipose tissue from freshly isolated aorta and trimming of branching arteries under the dissection microscope (left, middle left). Longitudinally split and pinned whole aorta before en-face staining (middle right). Enumeration of total aorta surface area and plaque surface area for morphometric analysis of Sudan-IV-stained whole aorta (right).