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. Author manuscript; available in PMC: 2016 Jun 28.
Published in final edited form as: Bio Protoc. 2016 Jun 5;6(11):e1833. doi: 10.21769/bioprotoc.1833

Figure 3. Atherosclerosis lesion analysis of aorta cross sections.

Figure 3

Freshly isolated aorta with thoracic and abdominal perivascular adipose tissue (left). Note that perivascular adipose tissue in aortic arch was removed for better visibility of the branching arteries. Schematic presentation of isolation and embedding of different aorta segments including thorax-I with its branches (cyan) and thorax-II (green) above diaphragm, abdomen-I with its branches (yellow) and abdomen-II with its branches (red) below diaphragm (middle). Rotate thorax-I at 180° angle before embedding. Arrow indicates direction of cutting. Enumeration of lumen area, internal elastic lamina (IEL) and external elastic lamina (EEL) area for morphometric analysis of Oil Red O and hematoxylin-stained innominate artery (right).