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. Author manuscript; available in PMC: 2016 Jun 28.
Published in final edited form as: Science. 2015 Aug 13;349(6252):1095–1100. doi: 10.1126/science.aac9373

Fig. 4. Complete biosynthesis of the opiate thebaine and semi-synthetic opioid drug hydrocodone in yeast.

Fig. 4

(A) Chromatograms of thebaine detected in CSY1064 media and 7.8 µg/L (25 nM) thebaine standard. (B) Spectra of eight multiple reaction monitoring (MRM) transitions of thebaine produced by engineered yeast and standard. (C) Chromatograms of hydrocodone detected in CSY1064+pCS2765 media and 0.3 µg/L (1 nM) hydrocodone standard. (D) Spectra of four MRM transitions of hydrocodone produced by engineered yeast and standard. Growth media was analyzed for opioids by LC-MS/MS MRM. Traces are representative of four biological replicates.

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