Table 1.
The primers sequences used for PCR
| Gene | Exon | Primer 5’-3’ | |
|---|---|---|---|
| Forward | Reverse | ||
| KRAS | 2 | GGTACTGGTGGAGTATTTGATAGTGT | GCAGGACCATTCTTTGATACAGA |
| 3 | CTTTGGAGCAGGAACAAT GTCT | GGGGAGGGCTTTCTTTGTGTA | |
| 4 | GTGTTACTAAT GACT GTGCTATAAC | GATTAAGAAGCAATGCCCTCTC | |
| NRAS | 2 | AT GTGGCT CGCCAAT TAACC | TCCGACAAGT GAGAGACAGGA |
| 3 | CACACCCCCAGGATTCTTACA | TCCTTT CAGAGAAAATAATGCTCCT | |
| 4 | CCCGTTTTTAGGGAGCAGA | GAATAT GGAT CACATCTCTACCAGAG | |
| PIK3CA | 9 | CCTGTCTCTGAAAATAAAGTCTTGC | AAAAGCATTTAATGTGCCAACGACC |
| 20 | TCGACAGCATGCCAAT CTCT | CT GAGAGTTAT TAACAGTGCAGT G | |
| BRAF | 15 | TCTTCATAAT GCTT GCTCT GATAGGA | CCCTGAGATGCTGCTGAGTT |