Fig 8. Quantitative RT-PCR of PLCe, Syt14, dlg5, and Mblk-1 in the worker brain during metamorphosis.

(A) Schematic drawing of the proliferation and differentiation of each KC subtype during the larval and pupal stages [32]. The lower bar indicates progression of the larval (L5F and L5S), prepupal (PP) and pupal stages (P0/1 to P9). The upper green, purple and red bars indicate the periods when the class-II KCs, lKCs, and sKCs are generated in the larval and pupal MBs. Black bar indicates the period when mKast expression is detected in the MBs. Dotted lines mean that the start points of proliferation of the class-II KCs, lKCs, and sKCs are obscure and that mKast expression was predicted but not analyzed previously [11]. L5F, fifth larval instar feeding stage; L5S, fifth larval instar spinning stage; PP, prepupa. (B-D) Change in the relative expression of PLCe (B), Syt14 (C), dlg5 (D), and Mblk-1 (E) during metamorphosis, respectively. Points and bars on each developmental stage indicate average +- standard deviation. Expression level of each gene normalized to that of RpL32 is indicated taking that at the P9 stage as 1. Three lots of samples were used for each developmental stage. n.d., not detected. For all genes, significant difference was detected by Kruskal-Wallis test (P < 0.05), whereas no significant difference was detected between expression levels at the adjacent stages (Steel-Dwass test, error rate was 0.05).