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. 2016 Mar 3;7(12):13269–13284. doi: 10.18632/oncotarget.7906

Figure 3. Ubiquitin proteasome-mediated degradation of WRN in CPT treated cells.

Figure 3

a. CPT-induced WRN down regulation is associated with Top1 degradation. Immunoblots showing WRN and Top1 protein levels in U2OS cells treated with indicated concentration of CPT for 16 h. b. Proteasome inhibitor MG132 inhibits the effect of CPT on WRN and Top1. U2OS cells were treated with 10 μM MG132 to inhibit proteasome pathway and with 10 μM E64 to inhibit lysosome pathway. c. Ubiquitination of WRN in CPT treated cells. Immunoblots showing ubiquitinated (HA-ubiqutin) 3xFlag-WRN in immuno­precipitated fractions of CPT (1μM) treated 293T cells expressing 3xFlag-WRN and HA-ubiqutin.