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. 2016 Feb 8;7(12):13634–13650. doi: 10.18632/oncotarget.7215

Figure 2. miR-335-5p and miR-15b-5p regulates TRIM29 expression at the post-transcriptional level.

Figure 2

A. miR-335-5p and miR-15b-5p expression levels were validated in snap-frozen human NPC (n = 25) and non-cancerous nasopharynx tissues (n = 17) by using quantitative real-time PCR analysis. MiR-335-5p and miR-15b-5p expression was significantly reduced in NPC than that in NP tissues (p < 0.001, independent Student's t-test). B. Predicted miR-335-5p and miR-15b-5p target sequences in the 3′UTRs of TRIM29. C. Relative luciferase activity of wt or mutant reporter plasmid co-transfected into 5-8F cells with miR-335-5p or miR-15b-5p mimics together with the negative control, or with miR-335-5p or miR-15b-5p inhibitors with the negative control. Cell lysates were obtained after 48h for analysis. Luciferase activity was normalized to that of the control group to obtain relative luciferase activity. “Wt” represents pMIR-wt-TRIM29-3′-UTR wild-type plasmid vector, and “mt” represents pMIR-mt-TRIM29-3′-UTR mutant reporter plasmid vector. Data were means ± SD (n = 3). Asterisks indicate values that are significantly different from the NC group (*P < 0.05, **P < 0.01). D. Representative immunoblots of TRIM29 protein expression after treatment with miR-335-5p and miR-15b-5p mimics or inhibitors in 5-8F cells.