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. 2016 Feb 11;7(12):13688–13705. doi: 10.18632/oncotarget.7327

Figure 7. CRP2 regulates MMP-9 expression in MDA-MB-231-luc cells.

Figure 7

A. CRP2 protein level in MDA-MB-231-luc cells transfected with a control siRNA (siCtr) or a CRP2 targeting siRNA (siCRP2). Forty hours after transfection, cells weretreated with or without PMA (100 ng/ml) in serum-free DMEM for 24 h. Average expression values (lower panel) were expressed as fold of CRP2 protein in untreated (−PMA) control siCtr cells which was set to 1. B. Gelatin zymography assays. The medium of the cell cultures described in A. was collected and assessed for its content in secreted pro-MMP-9. The results were expressed as fold of pro-MMP-9 secretion in PMA-treated control siCtr cells which was set to1. C. and D. Expression levels of cytosolic MMP-9 protein (C) and MMP-9 mRNA (D) in the cell cultures described in A. as assessed by western blot and real-time qPCR analyses, respectively. The results were expressed as fold of pro-MMP-9 expression in PMA-treated control siCtr cells which was set to 1. The data originate from at least 3 independent experiments. Error bars denote standard error. Significant levels: *: p < 0.01.

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