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. 2016 Feb 11;7(12):13706–13716. doi: 10.18632/oncotarget.7333

Figure 1. TAZ is activated by HRG1 in breast cancer cells.

Figure 1

(A) HRG1 induced increasing TAZ protein level in MCF7 cells. MCF7 cells were serum starved for overnight, then treated with 10 nM HRG1 with the indicated time. Cell lysates were processed for western blotting using antibodies against the indicated proteins. (B) HRG1 increased TAZ protein level and promoted TAZ nuclear localization. Immunofluorescent staining of TAZ in the serum starved and HRG1 treated MCF7 cells were performed. (C) Knockdown of TAZ reduced the expression of Hippo target genes induced by HRG1 in MCF7. Cells were transfected with indicated siRNA for two days and were changed to serum free medium for overnight. On the third day, samples were collected after 1 h HRG1 treatment. (D) Knockdown of TAZ blocked cell migration induced by HRG1. Cells were transfected with TAZ siRNA for two days and serum-starved for overnight. The cell migration was analyzed by transwell with or without HRG1 (10 nM) for 48 h. **P < 0.01 by Student's t-test.