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. 2016 Feb 11;7(12):13706–13716. doi: 10.18632/oncotarget.7333

Figure 2. Transcriptional regulation of TAZ by MRTF/SRF in breast cancer cells.

Figure 2

(A) HRG1 promoted TAZ transcription in MCF7 cells. qPCR analysis of TAZ mRNA level in the MCF7 cells after HRG1 treatment for the indicated time. (B) Inhibition of RhoA dependent transcription blocked the TAZ induction by HRG1 in MCF7 cells. MCF7 cells were serum starved for overnight. Before HRG1 treatment, the cells were pre-incubated with Latrunculin B and CCG-1423 for 1 h. TAZ mRNA levels were measured. (C) Western blot analysis of TAZ protein level in MCF7 cells treated with indicated compounds. (D) Knockdown of SRF suppressed induction of TAZ mRNA by HRG1 and Cytochalasin D in MCF7 cells. Cells were transfected with SRF siRNA for two days, then serum starved for overnight. HRG1 treatment was performed on the third day. (E) Western blot analysis of the TAZ protein level in the MCF7 cells with SRF knockdown and HRG1 treatment. (F) Knockdown of MRTF-A inhibited the TAZ induction by HRG1 in MCF7. Experiments were performed like SRF knockdown as described above.