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. 2016 Feb 13;7(12):13965–13975. doi: 10.18632/oncotarget.7369

Figure 3. 1 inhibits the interaction of p53/hDM2 in A375 cells without affecting protein expression levels.

Figure 3

(A) 1 inhibits the p53/hDM2 NanoBRET protein-protein interaction. (B) 1 inhibits the interaction of p53/hDM2 in A375 cells. The complex of p53/hDM2 in A375 cells was pulled down using anti-Flag magnetic beads and determined by probing with anti-Flag and anti-myc antibodies. (C) 1 has no significant effect on the protein expression levels of p53 and hDM2 in A375 cells. The protein expression levels of p53 and hDM2 determined by probing with anti-p53 and anti-hDM2 antibodies.