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. 2016 Feb 16;7(12):14336–14349. doi: 10.18632/oncotarget.7425

Figure 2. ZBTB20 facilitates proliferation and tumorigenicity of HCC cells.

Figure 2

(A and B) Comparing differences in the expression levels of ZBTB20 mRNA and protein between HCC cell lines with different proliferative potentials and the immortalized hepatic cell line. n = three repeats with similar results, *P < 0.05 and **P < 0.01 by ANOVA. (C) SMMC-7721 cells that were tranfected with empty vector (EV) or ZBTB20 retroviruses were subjected to immunoblotting for ZBTB20. ZBTB20 was knocked down by a specific siRNA and confirmed by Western blot in Hep3B cells. n = three repeats with similar results, **P < 0.01 by t test. (D) As assessed by MTT assays, ZBTB20 overexpression enhanced cell viability of SMMC-7721 cells and ZBTB20 knockdown was found to reduce Hep3B cell viability. n = three repeats with similar results, **P < 0.01 by ANOVA. (E) Cell proliferation as measured by BrdU incorporation assays was promoted by ZBTB20 overexpression in SMMC-7721 cells and suppressed by ZBTB20 knockdown in Hep3B cells. n = three repeats with similar results, **P < 0.01 by t test. (F) Representative colony formation assays were shown in HCC cells with altered ZBTB20 expression. Quantitative data disclosed that the ability of colony formation was enhanced after ZBTB20 overexpression in SMMC-7721 cells and reduced after ZBTB20 knockdown in Hep3B cells. n = three repeats with similar results, **P < 0.01 by t test.