Skip to main content
. 2016 Feb 16;7(12):14336–14349. doi: 10.18632/oncotarget.7425

Figure 3. ZBTB20 promotes cell cycle progression in HCC cells.

Figure 3

(A) SMMC-7721 cells that were transfected with empty vector (EV) or ZBTB20 were subjected to flow cytometry for cell cycle. ZBTB20 overexpression promoted cell cycle progression with decrease of cells in G0/G1 phase and increase of cells in S phase. n = three repeats with similar results, **P < 0.01 by t test. (B) Cell cycle as measured by flow cytometry after ZBTB20 knockdown showed an increase of Hep3B cells in G0/G1 phase and decrease of cells in S phase. n = three repeats with similar results, **P < 0.01 by t test. (C) ZBTB20 overexpression up-regulated the expressions of Cyclin D1 and Cyclin E, and down-regulated the levels of p21 and p27 in SMMC-7721 cells. n = three repeats with similar results, **P < 0.01 by t test. (D) Hep3B cells that were transfected with non-targeting (NT) or ZBTB20 siRNA were subjected to Western blot for Cyclin D1, Cyclin E, p21 and p27. n = three repeats with similar results, **P < 0.01 by t test.