Skip to main content
. 2016 Feb 16;7(12):14336–14349. doi: 10.18632/oncotarget.7425

Figure 7. FoxO1 knockdown inhibits cancer cell growth.

Figure 7

(A) SMMC-7721 cells that were transfected with non-targeting (NT) or FoxO1 siRNA were subjected to Western blot for FoxO1. n = three repeats with similar results, **P < 0.01 by t test. (B) As assessed by MTT assays, FoxO1 knockdown enhanced cell viability in SMMC-7721 cells. n = three repeats with similar results, **P < 0.01 by ANOVA. (C) SMMC-7721 cell proliferation as measured by BrdU incorporation assays was promoted by FoxO1 knockdown. n = three repeats with similar results, *P < 0.05 by t test. (D) The ability of colony formation was increased after FoxO1 knockdown in SMMC-7721 cells. n = three repeats with similar results, *P < 0.05 by t test. (E) FoxO1 knockdown promoted cell cycle of SMMC-7721 cell with decrease of cells in G0/G1 phase and increase of cells in S phase. n = three repeats with similar results, **P < 0.01 by t test. (F) FoxO1 knockdown up-regulated the expressions of Cyclin D1 and Cyclin E, and down-regulated the levels of p21 and p27 in SMMC-7721 cells. n = three repeats with similar results, *P < 0.05 and **P < 0.01 by t test.