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. 2016 Feb 11;7(12):14350–14365. doi: 10.18632/oncotarget.7320

Figure 4. IL-6 and SDF-1 reverse the effect on actin polymerization after LDE225 treatment.

Figure 4

A. Components in the FAK signaling pathway before or after LDE225 treatment (30 μM) with or without SDF-1 (100 ng/ml) and IL-6 (50 ng/ml) were analyzed in the SP53 cells and MCL patient cells using immunoblots. GAPDH was used as a loading control. B. Representative confocal microscopic images of MCL cells under different treatments. To visualize F-actin and the nucleus, cells were stained with FITC-phalloidin (green) and draq5 (blue), respectively. F-actin fluorescence (pointed with arrows) in cells treated with LDE225 (30 μM) was reduced compared to DMSO-treated cells. Combination of LDE225 with either SDF-1 (100 ng/ml) or IL-6 (50 ng/ml) reversed F-actin fluorescence to a nearly normal level. Scale bar, 10 μm. Control represents draq-5 stained cells without FITC-phalloidin staining.