Skip to main content
. 2016 Feb 20;7(12):14586–14604. doi: 10.18632/oncotarget.7542

Figure 3. MST3 interacts with VAV2 HEK293 cells were co-transfected with MST3-myc and HA-VAV2 or HA-VAV3.

Figure 3

A., B. and C. Equal amounts (0.5mg) of total protein were immunoprecipitated with 1μg anti-myc or anti-HA antibody. The immunoprecipitates were assessed by immunoblotting using anti-myc and anti-HA antibodies. D. Equal amounts (1mg) of total protein from MDA-MB-231 cells were immunoprecipitated with 1μg anti-MST3 antibody. The immunoprecipitates were assessed by immunoblotting using anti-MST3 or anti-VAV2 antibody. E. 0.5μg purified recombinant MST3 incubated with 1μg purified recombinant VAV2 in vitro. The protein mixtures were immunoprecipitated with anti-MST3 antibody. The immunoprecipitates were assessed by immunoblotting using anti-MST3 and anti-VAV2 antibody. F. Representative photographs for immunofluorescence staining of endogenous MST3 and VAV2 in MDA-MB-231 cells. Nuclei were stained with DAPI.