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. 2016 Feb 20;7(12):14586–14604. doi: 10.18632/oncotarget.7542

Figure 6. MST3 enhances VAV2 phosphorylation and Rac1 activation.

Figure 6

A. and C. MST3 shRNA was stably expressed in MDA-MB-231 and MDA-MB-468 cells. E. Wild-type-MST3-HA or mutant MST3-HA (deletion of proline rich domain; ΔP) was expressed in MDA-MB-468 cells. Equal amounts (30μg) of protein from whole-cell lysates were analyzed for VAV2, ectopic MST3 protein (HA), endogenous MST3, and β-actin expression by Western blotting analysis. 50μg total proteins from whole-cell lysates were analyzed by immunoblotting with antibody specific to phosphorylated VAV2. GTP-Rac1 was assayed using a Rac1 Activation Assay kit. B., D. and F. Quantification and statistical analysis of pVAV2 and GTP-Rac1. Data are represented as mean ± SD from three independent experiments. *p < 0.05; ** p < 0.01.