Skip to main content
. 2016 Feb 19;7(12):14693–14707. doi: 10.18632/oncotarget.7492

Figure 5. Effects of siRNA-mediated knockdown of Beclin-1, ATG5, or ATG7 on obatoclax-induced LC3-II accumulation.

Figure 5

(A) The efficacy of Beclin-1 (BECN1), ATG5, or ATG7 knockdown by respective siRNA was verified by Western blot analysis. Nontargeting siRNA was used as control siRNA, which has no homology to any known mammalian genes. Quantification of the ratios of Beclin-1/β-actin, ATG5/β-actin, and ATG7/β-actin is shown below each gel lane. The ratios were normalized to control siRNA-transfected cells. (B) After transfection with the control siRNA, Beclin-1 siRNA, ATG5 siRNA, or ATG7 siRNA, cells were treated with obatoclax at the concentration of 0.25 μM (EC109 and EC109/CDDP cells) and 0.125 μM (HKESC-1 and HKESC-1/cis cells) for 3 h. The conversion of LC3-I to LC3-II was determined by Western blot analysis. β-actin was used to evaluate protein loading. Blots were representative of 3 independent experiments. Quantification of the ratios of LC3-II/β-actin is shown below each gel lane. The ratios were normalized to control siRNA-transfected cells with obatoclax treatment.