Figure 8. Accumulation of polyubiquitinated proteins caused by obatoclax in the absence or presence of MG132.

Cells were treated with or without obatoclax or CQ at the indicated concentration in the absence or presence of MG132 for 48 h, and the amount of polyunbiquitinated proteins was examined by Western blot analysis. β-actin was used to evaluate protein loading. Blots were representative of 3 independent experiments.