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. 2016 Feb 17;7(12):14765–14780. doi: 10.18632/oncotarget.7430

Figure 4. Effects of LOX-1 downregulation in DLD-1 cells.

Figure 4

a. Time course analyses of DLD-1 cell proliferation. Newly infected cells after puromycin selection were plated in replicates and daily counted. Growth curve of LOX-1RNAi and scrambleRNAi DLD-1 cells was performed. The data represent the average ± S.D. of three separate experiments(*P <0.05). b. Wound-healing/invasion assay performed on LOX-1RNAi and scrambleRNAi DLD-1 cells. After mechanically scratching cell monolayer, the timing of filling the gap was observed. Images were captured at 0 hour (T0), 24 hours(T1), 48 hours (T2) and 72 hours (T3) post wounding. The gap was indicated by a black bar. c. LOX-1 neutralization was performed by using a commercial antibody. Following 5 days, cells (DLD-1 LOX-1Ab) were collected, counted and compared to control ones (DLD-1). The assay reveals a strong reduction in DLD-1 cells proliferation (DLD-1 LOX-1Ab) compared to DLD-1 untreated cells (*P<0.05).