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. 2016 Feb 17;7(12):14765–14780. doi: 10.18632/oncotarget.7430

Figure 6. H4 acetylation immunocytochemistry and sodium Butyrate treatment.

Figure 6

a. H4 acetylation was evaluated by immunocytochemistry comparing scrambleRNAi and (b) LOX-1RNAi DLD-1 cells. A strong increase of nuclear signal was observed in LOX-1RNAi DLD-1 cells. Cells were light counterstained with eosin. 40X magnification. c. Na Butyrate treatment in scrambleRNAi and LOX-1RNAi DLD-1 cells at 24 and 48 hours. The proliferation rate of scrambleRNAiDLD-1 cells-treated with Na Butyrate- resulted to be slowed down both after 24 and 48 hours. Na Butyrate treatment to LOX-1RNAi DLD-1 cells induced after 48 hours an evident decrease in term of proliferation rate respect to untreated ones.