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A. PBMC were cocultured with tumor cells lines (HepG2, SK-Hep-1, Hep3B) or normal liver cells (L02), and the production of cytokine (TNF-α, IFN-γ, IL-6, IL-10) was measured by ELISA. B. HepG2 cocultured with PBMC were treated with indicated blocking Abs or control IgG. The expression of IDO1 in HepG2 was determined by western blot and ELISA.
