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. 2016 Feb 22;7(12):15047–15064. doi: 10.18632/oncotarget.7592

Figure 4. Impact of the FAAH inhibitors AA-5HT and URB597 on tumor cell invasion and TIMP-1 expression of A549 cells.

Figure 4

A, C. Concentration-dependency of FAAH inhibitors' anti-invasive action (black bars) and its impact on cell viability (open bars) and migration through uncoated Boyden chambers (grey bars). B, D. Concentration-dependent effect of FAAH inhibitors on TIMP-1 protein levels. A549 cells were incubated with vehicle, AA-5HT or URB597 for 72 h before measurement of invasion or migration using Boyden chamber assays (A, C), WST-1 test (A, C) or Western blot analyses of TIMP-1 from cell culture media (B, D). Protein staining of cell culture media is shown as loading control (LC). All values are expressed as percent control in comparison with vehicle-treated cells (100%) in the absence of test substance. Data are means ± SEM of n = 5 (A, invasion) or n = 4 (A,C migration and viability; B, blots), n = 11-12 (C, invasion) or n = 7 (D, blots) experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle; one-way ANOVA plus post hoc Dunnett test.