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. 2016 Feb 23;7(12):15093–15104. doi: 10.18632/oncotarget.7618

Figure 2. Effect of miR-15a and miR-16 on proliferation, clonal growth and chemosensitivity in OvCa.

Figure 2

A. OVSAHO or CP20 cells were transfected with miR-CTL, miR-15a, miR-16 or miR-15a+ miR-16. 24h after transfection, cells were counted and re-plated in 96-well plate and relative proliferation was evaluated using the CyQUANT® NF Cell Proliferation Assay Kit at indicated time points B. OVSAHO and CP20 cells were transfected with respective microRNAs, additionally CP20 cells were also transfected with the Flag-BMI1 construct that is non-responsive to the microRNAs. 48 h after transfection cells were re-plated in 0.3% agar and remaining cells subjected to immunoblotting for BMI1 and Flag, shown in the inset. After 10 (CP20) or 14 (OVSAHO) days, colonies were stained with crystal violet, imaged and 9 images from 3 independent experiments were quantified using ImageJ in a blinded fashion. C. OVSAHO and CP20 cells were transfected with miR CTL, miR-15a, miR-16 or miR-15a+ miR-16 for 24h and cells re-plated in 96-well plates. Cells were treated with cisplatin for 48h at the indicated concentrations and viability was determined using CyQUANT® NF Cell Proliferation Assay. Percent cell viability was evaluated by comparing with respective vehicle (V) treated cells. Data represent mean ± SD. *, P<0.05.