Table 1. Experimental design and time table for treatment.
Days | |||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2 | 1 | 0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |
ALA | √ | √ | √ | ||||||||||||||
Radiation | √ | ||||||||||||||||
Sampling | √ | √ | √ |
Thirty-nine BALB/c mice were randomized to four groups: i) control group (9 mice), ii) ALA group (9 mice), iii) RT group (11 mice), and RT + ALA group (10 mice). Small intestine damage was induced by irradiation (15 Gy, 1 fraction) on the abdomen. In the ALA group, mice received 100 mg/kg/day ALA by intraperitoneal injection on days −2 to 0. The control group received vehicle in the same way. The small intestine was collected 3rd, 7th, and 14th days after irradiation.