Table 1.
Summary of published techniques for multiplexed measurements of multiple substrates/products
| Subjects studied | Techniques for measurements | Categories of studies | Reference (year) |
|---|---|---|---|
|
| |||
| Multiple PAH biodegradation | HPLC | Monod kinetics | [30] (1988); [31] (1999) |
| Glycogen synthase for adenine- and uridine-diphosphate glucose polymerization | MS | Double-reciprocal kinetic | [33] (2003) |
| Sugar nucleotidyltransferases for multiple substrates | Michaelis-Menten kinetics | [34] (2005) | |
| NodST, a GlcNAc-6-O-sulfotransferase, for four chitoologosaccharides synthesis | Internal competition kinetics | [38] (2004) | |
| Gcn5-mediated histone H3 lysine acetylation and nonenzymatic acetylation | Site specific internal competition kinetics | [7] (2013) | |
| Comparison of Rtt109-Vps75 mediated histone acetylation on different histone complexes | Site specific internal competition kinetics | [14] (2015) | |
| P300 and CBP for histone H3 and H4 acetylation | Site specific internal competition kinetics | [41] (2013) | |
| DNA methylation | Quantification of nucleosides | [73] (2008) | |
| tRNA modifications | Quantification of nucleosides | [74] (2010); [75] (2014) | |
|
| |||
| Sialidase hydrolysis of Neu5Acα2, 6LacβSPh (12C/13C and 16O/18O) | NMR | Competitive kinetic isotope effects | [28] (2010) |
| Catalysis by thymidylate synthase | Kinetic isotope effects and hydrogen tunneling | [51] (2004) | |
| Catalysis by dihydrofolate reductase | Kinetic isotope effects and hydrogen tunneling | [52] (2011) | |
|
| |||
| Mechanism of mutant and wild type T4 DNA polymerases (3H and 14C) | Radiolabeling | Internal competition kinetics | [53] (1979) |
| Steady state and pre-steady state kinetics for studying DNA polymerase fidelity (32P) | Internal competition kinetics | [56] (2010) | |
| Kinetics of ribonuclease P over 80 competing tRNA precursors (32P) | Internal competition kinetics | [58] (2013) | |
| Modeling of ribonuclease P processing 3 competing tRNA precursors (32P) | Internal competition kinetics | [59] (2014) | |
| Specificity study on ribozyme for variable substrate sequences (32P) | Individual kinetics | [60] (1988) | |
| Ribonuclease L cleavage sites in viral RNAs (32P) | Cleavage site study | [68] (2004) | |
| Serial analysis of mutation spectra (32P) | Identification of DNA damage sites | [69] (2008) | |
|
| |||
| Proofreading efficiency of wild type T4 DNA polymerase | Fluorescence | Internal competition kinetics | [54] (1994) |
| Cleavage rate study on 8–17 deoxyribozyme | Internal competition kinetics | [55] (2010) | |
|
| |||
| Ribonuclease L cleavage sites in host (HeLa cell) and viral RNAs | Deep sequencing method | Characterization of cleavage sites | [66] (2014) |
| Ribonuclease L cleavage sites in viral RNAs | Characterization of cleavage sites | [67] (2014) | |
|
| |||
| Cytokine gene expression in domestic mammals | Quantitative competitive PCR | Internal competition | [78] (1996) |