(A) Left, representative photo of GFP expression in L6 infected with Lv-shRNA-Smad4 and the uninfected L6 cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. (B) Left, representative photo of GFP expression in RSC-364 infected with Lv-shRNA-Smad4 and the uninfected RSC-364 cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. (C) Left, representative photo of GFP expression in RC infected with Lv-shRNA-Smad4 and the uninfected RC cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. ** P < 0.01 compared to the Lv-NC group.