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. 2016 Jun 28;11(6):e0158093. doi: 10.1371/journal.pone.0158093

Fig 1. Lentiviral mediates Smad4 knockdown in L6, RSC-364 and RC cells.

Fig 1

(A) Left, representative photo of GFP expression in L6 infected with Lv-shRNA-Smad4 and the uninfected L6 cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. (B) Left, representative photo of GFP expression in RSC-364 infected with Lv-shRNA-Smad4 and the uninfected RSC-364 cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. (C) Left, representative photo of GFP expression in RC infected with Lv-shRNA-Smad4 and the uninfected RC cells for gene delivery efficiency assessment 72 hours after infection. Right, results of Smad4 mRNA quantification. ** P < 0.01 compared to the Lv-NC group.