Table 2. List of primers for the amplification and resequencing of the newly developed markers.
Marker-name | Primer | Sequence 5‘-3‘ | length (bp) | Annea-ling (°C) | Acces. no. |
---|---|---|---|---|---|
psaI-ycf4 | psaI | CGTGTGTAAACATGATATATGAG_FAM | 174- | 55 | KU2010 |
ycf4 | GAGTAATTCATCGAATTGGTTAG | 178 | 20–22 | ||
psbE-petL | psbE | AAGGAATTGGTTAGTTGTCCAG_VIC | 179- | 55 | KU2010 |
petL | TTACATATCTTAAATTAGAGAGCC | 185 | 23–25 | ||
trnLF | trnL1 | CAATACATATCATTTCTTGTACTG_PET | 130- | 53 | KU2010 |
trnF2 | TAGATAACTTGAGTTTATGTCAATT | 135 | 26–28 | ||
trnCD | trnC5 | TTGGATAGACGAACGGGGAAT_FAM | 115- | 57 | KU2010 |
trnD5 | TATCATATTAAATTGATTGCCGG | 123 | 29–31 | ||
trnDT | trnD3 | GGATAGGGATCAACAAGTTATTG_PET | 187- | 52 | KU2010 |
trnT4 | CAAGACCGACCCTAATTGAAT_PET | 189 | 32–34 |