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. 2016 Jun 28;11(6):e0158221. doi: 10.1371/journal.pone.0158221

Table 2. List of primers for the amplification and resequencing of the newly developed markers.

Fluorescent-labeling of the primers is given in column “sequences”: FAM = blue, VIC = green, PET = red. In the last column, the accession numbers of the related markers for the three species Q. robur, Q. mongolica and Q. alba are given. “Length” means sequence length.

Marker-name Primer Sequence 5‘-3‘ length (bp) Annea-ling (°C) Acces. no.
psaI-ycf4 psaI CGTGTGTAAACATGATATATGAG_FAM 174- 55 KU2010
ycf4 GAGTAATTCATCGAATTGGTTAG 178 20–22
psbE-petL psbE AAGGAATTGGTTAGTTGTCCAG_VIC 179- 55 KU2010
petL TTACATATCTTAAATTAGAGAGCC 185 23–25
trnLF trnL1 CAATACATATCATTTCTTGTACTG_PET 130- 53 KU2010
trnF2 TAGATAACTTGAGTTTATGTCAATT 135 26–28
trnCD trnC5 TTGGATAGACGAACGGGGAAT_FAM 115- 57 KU2010
trnD5 TATCATATTAAATTGATTGCCGG 123 29–31
trnDT trnD3 GGATAGGGATCAACAAGTTATTG_PET 187- 52 KU2010
trnT4 CAAGACCGACCCTAATTGAAT_PET 189 32–34