Table 4. PCR conditions compared for leaf and timber.
Leaf | Timber | |||||||
---|---|---|---|---|---|---|---|---|
PCR cycles | Conc. MgCl2 | Enhancer | Conc. Primer | PCR cycles | Conc. MgCl2 | Enhancer | Conc. Primer | |
psaI-ycf4 | 30 | 1.75 mM | no | 0.07 μM | 40 | 2.0 mM | no | 0.1 μM |
psbE-petL | 30 | 1.75 mM | no | 0.05 μM | 40 | 2.0 mM | no | 0.05 μM |
trnLF | 30 | 1.75 mM | yes | 0.2 μM | 40 | 2.0 mM | yes | 0.2 μM |
trnCD | 25–30 | 1.5 mM | yes | 0.13 μM | 35 | 2.5 mM | yes | 0.3 μM |
trnDT | 25 | 1.75 mM | yes | 0.1 μM | 40 | 2.0 mM | yes | 0.1 μM |