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. 2016 Jun 28;11(6):e0158221. doi: 10.1371/journal.pone.0158221

Table 4. PCR conditions compared for leaf and timber.

Only the differences are shown, all other parameters are as given in material and methods.

Leaf Timber
PCR cycles Conc. MgCl2 Enhancer Conc. Primer PCR cycles Conc. MgCl2 Enhancer Conc. Primer
psaI-ycf4 30 1.75 mM no 0.07 μM 40 2.0 mM no 0.1 μM
psbE-petL 30 1.75 mM no 0.05 μM 40 2.0 mM no 0.05 μM
trnLF 30 1.75 mM yes 0.2 μM 40 2.0 mM yes 0.2 μM
trnCD 25–30 1.5 mM yes 0.13 μM 35 2.5 mM yes 0.3 μM
trnDT 25 1.75 mM yes 0.1 μM 40 2.0 mM yes 0.1 μM