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. 2016 Jun 28;11(6):e0158438. doi: 10.1371/journal.pone.0158438

Fig 8. Effect of CREB, PKCδ, and PKCμ/PKD KD on U46619-induced signaling and CXCL1/8 expression.

Fig 8

(A) Effect of PKCδ/θ and PKCμ/PKD KD on U46619-induced signaling: Cells were transfected with control or PKCδ and PKCμ/PKD siRNA for 72 h and followed by addition of vehicle or U46619 (2 μM) for 120 min. Protein expression and phosphorylation was determined through Western blotting. Two representative results were shown from four independent results. (B) Effect of PKCμ/PKD or CREB KD on U46619-induced CXCL1/8 expression: Cells were transfected with control, PKCμ/PKD, or CREB siRNA for 72 h and followed by addition of vehicle or U46619 (2 μM) for 16 h. CXCL1 and CXCL8 expression was determined through ELISA (n = 3–4). *P < 0.05, **P < 0.01 versus control.