Fig 1. Identification and prediction of putative leucine-based motifs in the N-terminal tail of OSTβ.

(A) Sequence of alignment of full-length OSTβ from different species. A highly conserved acidic dileucine motif (ELLEE) was identified within the N-terminal region of all species. The stars “**” indicate a dileucine motif at position L20L21. The predicted TM domain is boxed. “-” denotes that the part of the sequence is skipped. (B) Prediction of the location of secondary structure at OSTβ extracellular and transmembrane (TM) domains. The prediction classifies each amino acid residue as belonging to alpha helix ('H') or beta sheet ('E') using a neural network called Jnet.