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a) The relative hydrolytic activities of PmEst on substrates with different acyl chain sizes were calculated with respect to C4. b) Determination of the optimum temperature of PmEst on pNB, and c) Residual activity normalized as a function of pre-incubation time at 25, 35, 45 and 55°C. PmEst was inactive when pre-heated at temperatures higher than 55°C. Error bars represent the standard deviations from the triplicate measurements.
