Fig 1. Synthetic mycolactone derivatives used for the generation and detection of mycolactone specific antibody responses.

PG-203 (A) was conjugated to BSA via the amino group on its diethylene glycol-based linker and the carrier protein conjugate was used for the immunization of mice. PG-204 (C) and PG-183 (D) are biotinylated mycolactone derivatives used for analytical purposes. Unmodified synthetic mycolactone A/B (B) was used as effector molecule in cytotoxicity assays.