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. 2016 Jun 28;10(6):e0004808. doi: 10.1371/journal.pntd.0004808

Fig 4. Effect of structural modifications of mycolactone on antibody binding.

Fig 4

Competition ELISAs were performed by coating microtiter plates with the mAbs and pre-incubation with serial dilutions of mycolactone A/B, natural mycolactone variants harboring modifications in the lower fatty acid acyl side chain (mycolactone C and mycolactone F), a truncated core molecule without O-linked lower side chain (PG-119) or derivatives containing substitutions in the upper side chain (PG-165, PG-157 and PG-182). Biotinylated PG-204 was subsequently added and its binding was detected by using alkaline phosphatase-conjugated streptavidin as reporter. Detailed results with all tested mycolactone derivatives are shown for mAb 5.1 as example.