Fig 7. Toxin neutralizing activity of anti-mycolactone mAbs.

L929 fibroblasts were incubated with 15 ng/ml (20 nM) synthetic mycolactone A/B and mAbs were added in different molar ratios of excess antibody versus mycolactone. After 48 h, the metabolic activity of fibroblasts was measured. The graph shows mean values across duplicate samples, and the error bars represent the standard deviation of three independent experiments. As control, the isotype-matched antibody JD4.1 was used.