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. 2016 Jun 28;10(6):e0004808. doi: 10.1371/journal.pntd.0004808

Fig 8. Inhibition of antibody mediated neutralization by addition of the non-toxic mycolactone core variant PG-119.

Fig 8

L929 cells were treated with a mix of 15 ng/ml (20 nM) synthetic mycolactone A/B and a 2.5 fold molar excess of the anti-mycolactone mAb JD5.1. Serial dilutions of PG-119 were added to the mix, starting with a PG-119 concentration of 40 ng/ml (86 nM). As control, the isotype-matched antibody JD4.1 was used. After 48 h, the metabolic activity of fibroblasts was measured and plotted against the concentration of PG-119. The graph shows mean values across duplicate samples, and the error bars represent the standard deviation of three independent experiments.