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. Author manuscript; available in PMC: 2016 Jun 28.

Published in final edited form as: J Am Chem Soc. 2016 Jan 21;138(4):1196–1205. doi: 10.1021/jacs.5b09259

Dependence of k_PCET on buffer concentration for photoβ₂s alone ( ), or in the presence of wt-α₂ ( ), or Y₇₃₁F-α₂ ( ). k_PCET was calculated according to Eq. 2. Triplicate sets of independently prepared samples contained 10 μM Y₃₅₆- or Y₃₅₆F-photoβ₂, 25 μM wt-α₂ or Y₇₃₁F-α₂, 1 mM CDP, 3 mM ATP in assay buffer containing 15 mM MgSO₄, 1 mM EDTA, and 50, 100, 150, or 500 mM HEPES buffer adjusted to pH 7.6.

Inline graphic — Dependence of k_PCET on buffer concentration for photoβ₂s alone ( ), or in the presence of wt-α₂ ( ), or Y₇₃₁F-α₂ ( ). k_PCET was calculated according to Eq. 2. Triplicate sets of independently prepared samples contained 10 μM Y₃₅₆- or Y₃₅₆F-photoβ₂, 25 μM wt-α₂ or Y₇₃₁F-α₂, 1 mM CDP, 3 mM ATP in assay buffer containing 15 mM MgSO₄, 1 mM EDTA, and 50, 100, 150, or 500 mM HEPES buffer adjusted to pH 7.6.