Table 3.
Solvent kinetic isotope effect on kPCET
| Interface residues | τ (ns)a | kPCET (105 s−1)b | SKIE | |||
|---|---|---|---|---|---|---|
|
| ||||||
| β356 | α731 | 1H | 2H | 1H | 2H | |
| Y | – | 410 (20) | 568 (5) | 8 (1) | 5.5 (2) | 1.5 (2) |
| F | – | 615 (5) | 827 (9) | |||
| Y | Y | 522 (8) | 713 (3) | 4.8 (3) | 3.2 (1) | 1.4 (1) |
| F | Y | 696 (3) | 920 (2) | |||
| Y | F | 578 (9) | 778 (6) | 3.6 (3) | 2.4 (1) | 1.5 (1) |
| F | F | 728 (5) | 952 (8) | |||
a
Triplicate sets of independently prepared samples contained 10 μM Y356 or Y356F-photoβ2, 25 μM α2 or Y731F-α2, 1 mM CDP, 3 mM ATP in assay buffer at pL 7.6.
b
Calculated according to Eq. 2.