Table 2. Anion versus cation permeability through WT EAAT1 and R477H.

Ion	Concentration	Erev
		EAAT1			R477H
		pH 5.5	pH 8.5		pH 5.5	pH 8.5
	mM	mV	mV		mV	mV
Na+	10	18 ± 7	37 ± 4		−19 ± 3	−52 ± 4
	100	21 ± 4	26 ± 3		−6 ± 2	−19 ± 2
Cl−	10	45 ± 3	52 ± 2		18 ± 3	−20 ± 3
	100	11 ± 2	34 ± 3		−9 ± 1	−18 ± 3

Reversal potential measurements (E_rev) for WT EAAT1 and R477H l-glutamate elicited currents at a range of Cl⁻ and Na⁺ concentrations at pH 5.5 and 8.5. Choline-Cl⁻ and Na⁺-gluconate were used to obtain correct osmotic balance. Data shown represent the mean ± SEM (n ≥ 4).