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. 2016 Jun 27;213(7):1185–1199. doi: 10.1084/jem.20151110

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© 2016 Keller et al.

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Figure 3. — TCR-induced signaling in LAT^mut T cells. (A–D) All experiments were performed in LAT-deficient J.CaM2.5 cells and J.CaM2.5 cells reconstituted with LAT^wt or LAT^mut. (A) Immunoblot of ZAP70 and PLCγ1 phosphorylation with or without stimulation with 5 µg/ml anti-CD3 for 3 min. (B) Phosphorylation of ITK (pITK) with or without stimulation with 5 µg/ml anti-CD3 for 2 min. (C) Ca²⁺ mobilization after anti-CD3 stimulation. (D) Phosphorylation of ERK (pERK) after 2-min anti-CD3 stimulation and up-regulation of CD69 after overnight stimulation. All results are representative of three to five independent experiments.