Table 2. JEV peptides eliciting T cell responses identified in this study.
| Subject | Peptide | Subset | JEV protein | Amino acid location | Type | HLA restriction | HLA class I | HLA class II | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | DR | |||||||||
| JE082/2 | GKNRAINMLKRGLPRVF | CD4 | C | 9–25 | JE | 11:01, 33:01 | 13:01, 44:02 | DR12, DR14, DR52 | ||||
| VFPLVGVKRVVMSLLDGR | CD4 | C | 24–41 | |||||||||
| JE093/2 | MLKRGLPRVFPLVGVKRVVMSLLDGR | CD4 | C | 16–41 | JE | 24:02, 24:02 | 13:01, 27:02 | DR15, DR51 | ||||
| JE043/2 | SLVNKKEAWLDSTKATRY | CD8 | M | 230–247 | JE | 01:01, 11:01 | 52:01, 57:01 | DR3, DR15, DR52, DR51 | ||||
| WIIRNPGYAFLAAVLGWM | CD4 | M | 254–271 | |||||||||
| JE101/2 | RYLMKTENWIIRNPGYAF | CD4 | M | 246–263 | JE | 30:01, 33:01 | 13:01, 44:02 | DR7, DR53 | ||||
| JE017/2 | AKFSCTSKAIGRTIQ | CD4 | E | 411–425 | JE | 03:01, 11:02 | 07:02, 35:01 | DR4, DR11, DR52, DR53 | ||||
| KQSVVALGSQEGGLHQAL | CD4 | E | 543–560 | |||||||||
| CRLKMDKLALKGTTYGMCTEKFSF | CD4 | E | 581–604 | |||||||||
| HGTVVIELSYSGSDGPCK | CD4 | E | 613–630 | |||||||||
| LGKAFSTTLKGAQRLAAL | CD4 | E | 697–714 | |||||||||
| WDFGSIGGVFNSIGKAVHQVFGGAF | CD4 | E | 719–735 | |||||||||
| GVLVFLATNVHADTGCAI | CD4 | E | 783–801 | |||||||||
| H021/1 | PWTSPSSTAWRNRELLM | CD4 | E | 518–534 | H | 02:01, 11:01 | 07:02, 52:01 | DR15, DR51 | ||||
| JE003/2 | KYLPETPRSLAKIVHK | CD4 + CD8 | NS1 | 827–842 | JE | 03:01, 33:01 | 15:01, 37:01 | DR10, DR15, DR51 | ||||
| AVHSDLSYWIESRYNDTWKLERAVF | CD4 | NS1 | 987–1011 | |||||||||
| WIESRYNDTWKLERAVFGEVKSCTW | CD4 | NS1 | 995–1019 | |||||||||
| JE001/2 | EAWVDRYKYLPETPRSLAKIVHK | CD4 | NS1 | 820–842 | JE | 11:01, 31:01 | 35:01, 52:21 | DR4, DR15, DR53, DR51 | ||||
| TYALNTFTNIAVQLVRLM | CD8 | NS5 | 3136–3153 | |||||||||
| H014/3 | TAVLAPTRVVAAEMAEAL | CD8 | NS3 | 1723–1740 | H | 03:01, 30:01 | 07:02, 13:01 | 06:02, 07:01 | ||||
| H001/1 | ALRGLPVRY | CD8 | NS3 | 1739–1747 | H | 11:01, 32:01 | 15:01, 55:01 | 01:02, 04:01 | ||||
| H001/4 | DVMCHATL | CD8 | NS3 | 1763–1770 | D | B*08:01 | 01:01, 03:01 | 08:01, 44:02 | 05:01, 07:01 | DR8, DR17, DR52 | ||
| H019/1 | NLFVMDEAHFTDPASI | CD8 | NS3 | 1784–1799 | H | 02:01 | 38:01, 40:06 | 07:01, 15:02 | ||||
| EAHFTDPASIAARGYI | CD8 | NS3 | 1790–1805 | |||||||||
| H013/3 | NLFVMDEAHFTDPASI | CD4 | NS3 | 1784–1799 | H | 02:01, 24:02 | 35:03, 40:06 | 02:21, 15:02 | ||||
| EAHFTDPASIAARGYI | CD4 + CD8 | NS3 | 1790–1805 | |||||||||
| GEAAAIFMT | CD8 | NS3 | 1812–1820 | |||||||||
| JE098/2 | ELGEAAAIFMTATPP | CD8 | NS3 | 1810–1824 | JE | 02:01 | 35:01, 40:06 | 04:01, 07:01 | ||||
| H005/1 | GCGRGGWSYYAATLKKV | CD8 | NS5 | 2608–2624 | H | 02:01, 30:01 | 13:01, 38:01 | 06:02, 12:02 | ||||
| RGYTKGGAGHEEPMLM | CD8 | NS5 | 2628–2643 | |||||||||
| H010/4 | LMQSYGWNL | CD8 | NS5 | 2642–2650 | D | 01:01, 02:01 | 08:01, 35:01 | 04:01, 07:01 | ||||
| LVSLKSGVDVFYKPS | CD8 | NS5 | 2650–2664 | |||||||||
| YMPKVIEKMEV | CD8 | NS5 | 2714–2724 | |||||||||
| NMTSQVLLGRMDRTVWR | CD8 | NS5 | 2761–2777 | |||||||||
| HPYRTWTY | CD8 | NS5 | 2827–2834 | B*35:01 | ||||||||
| TPFGQQRVF | CD4 + CD8 | NS5 | 2873–2884 | |||||||||
| JE080/2 | KLNAMSREEFFKYRREAIIEVDR | CD4 | NS5 | 2542–2564 | JE | 02:01, 33:01 | 15:05, 44:02 | DR7, DR53 | ||||
| TWHKDPEHPYRTWTYHGSYEVK | CD4 | NS5 | 2820–2841 | |||||||||
| H007/2 | TMAMTDTTPFGQQRVFK | CD4 + CD8 | NS5 | 2869–2885 | H | 01:01, 68:01 | 57:01 | DR53, DR7 | ||||
| H011/3 | TMAMTDTTPFGQQRVFK | CD8 | NS5 | 2869–2885 | H | 02:01, 68:01 | 37:01, 51:01 | 06:02, 15:02 | ||||
| H007/3 | TMAMTDTTPFGQQRVFK | CD4 + CD8 | NS5 | 2869–2885 | H | 11:01, 68:01 | 07:02, 52:02 | 07:01, 12:02 | ||||
| CTKEEFIKKVNSNAAL | CD8 | NS5 | 2923–2938 | |||||||||
| DIAGKQGGKMYADDTAGW | CD4 | NS5 | 3050–3067 | |||||||||
| H002/3 | IWFMWLGARY | CD8 | NS5 | 3003–3012 | H | 01:01, 29:01 | 07:05, 57:01 | 06:02, 15:04 | ||||
| RYLEFEALGFL | CD4 | NS5 | 3011–3021 | |||||||||
| JE054/2 | AISGDDCVVKPLDDRFATALHFL | CD8 | NS5 | 3191–3213 | JE | 11:01, 24:02 | 15:25, 51:01 | 07:01, 16:01 | ||||
| JE074/2 | CVVKPLDDRFATALHFL | CD8 | NS5 | 3197–3213 | JE | |||||||
| H008/4 | MTTEDMLQVW | CD4 + CD8 | NS5 | 3336–3345 | H | B*58:01 | 01:01, 33:03 | 58:01, 15:17 | 07:01, 03:04 | DR13, DR15, DR51, DR52 | ||
| CSAVPVDW | CD8 | NS5 | 3312–3319 | B*58:01 | ||||||||
| JE446/2 | GEWMTTEDMLQVWNRVWI | CD4 | NS5 | 3333–3350 | JE | 01:01, 33:01 | 52:01, 58:01 | DR14, DR15, DR52, DR51 | ||||
| MLQVWNRVWIEENEWMM | CD8 | NS5 | 3341–3357 |
JE, recovered JE patient; H, healthy JEV-exposed donor; D, dengue exposed. In total, 57 responses to 40 peptides (or adjacent pairs of peptides) were identified.