Figure 1.

One-carbon folate metabolism is a core pathway altered with AML targeting compounds. (A) Intersection of genes down-regulated by five compounds that cause AML death and differentiation: Vitamin D, PMA, ATRA, JQ1, and EPZ004777. Reported is the number of significantly down-regulated genes by each agent (DMSO vs. agent: SNR ≥ 1.5; P ≤ 0.05; FDR[BH] ≤ 0.05). The core 198 genes are significantly down-regulated by each of the five agents (DMSO vs. agent: average SNR across all conditions ≥ 1.5 and SNR ≥ 1.5 in four out of five conditions). P-values calculated using the permutation test implemented in the comparative marker selection procedure, Gene Pattern v3.8.1. (B) Pathway enrichment analysis across KEGG canonical pathways showed eight significantly overlapping pathways. P-values calculated using the hypergeometric test for gene set overlapping analysis. (C) Heat maps showing expression for genes of one-carbon folate pathway across five treatments with differentiation agents. (D) Expression of MTHFD2 across the hematopoietic lineage.