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Order, amounts, purities and molecular weights of reagents for preparing 1000 ml of 1.5 SBF.
| Order | Reagent | Amount | Purity (%) | Molecular weight |
|---|---|---|---|---|
| 1 | NaCl | 12.0525 g | 99.5 | 58.4430 |
| 2 | NaHCO3 | 0.5325 g | 99.5 | 84.0068 |
| 3 | KCl | 0.3375 g | 99.5 | 74.5515 |
| 4 | K2HPO4 · 3H2O | 0.3465 g | 99.0 | 228.2220 |
| 5 | MgCl2 · 6H2O | 0.4665 g | 98.0 | 203.3034 |
| 6 | 1.0 M-HCl | 58.5 ml | – | – |
| 7 | CaCl2 | 0.438 g | 95.0 | 110.9848 |
| 8 | Na2SO4 | 0.108 g | 99.0 | 142.0428 |
| 9 | Tris | 9.177 g | 99.0 | 121.1356 |
| 10 | 1.0 M-HCl | 0–8 ml | – | – |
