Figure 2. CMEC media decreases PBMC viability.

(A) PBMCs were grown under selected media conditions at 1 x 10⁶ cells/mL at 37°C in 5% CO₂ for 24 hours prior to an MTS assay to assess cellular viability. Media conditions: complete PBMC media; complete CMEC media; CMEC media supplemented with IL-2; combination of one part PBMC and one part CMEC; combination of one part PBMC and three parts CMEC; three parts PBMC and one part CMEC. PBMC viability within complete PBMC media was normalized to 100%, and all further media conditions were compared to complete PBMC media. (B) PBMCs were grown as in (A). Media conditions included either an RPMI or EBM-2 media base with the addition of selected components of complete CMEC media. HC – hydrocortisone, AA – ascorbic acid, CDLC – chemically defined lipid concentrate, and bFGF – basic fibroblast growth factor. All media conditions included IL-2 and Pen/Strep. An MTS assay was performed to assess PBMC viability. PBMC viability within complete PBMC media (condition 1) was normalized to 100%, and all further media conditions were compared to complete PBMC media (condition 1). Media formulations and components highlighted in orange indicate conditions which yielded less than 75% viability. All conditions with less than 75% viability contain either EBM-2 or HC. (C) Percent PBMC viability as determined by MTS assay. Conditions refer to those defined in (B). Black line: 90%. Red line: 75%.