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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: J Neurosci Methods. 2016 May 20;269:39–45. doi: 10.1016/j.jneumeth.2016.05.016

Figure 3. Co-culture media does not induce leakiness of the hCMEC/D3 barrier.

Figure 3

The effect of co-culture media on hCMEC/D3 structure and function was assessed through a FITC-D permeability coefficient assay as previously described (Figure 1). Media conditions: complete CMEC media; CMEC media without HC; CMEC media made with an RPMI base (no EBM-2); CMEC media without HC made with an RPMI base. A 30 minute treatment with mannitol (1.4 M) is included as a positive control of barrier compromise for the assay. Statistical analysis was performed with a two-tailed Student’s t-test. *p = 0.04

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