Table 4.
Adipose tissue leukocyte numbers at baseline (pre-intervention) and after 7% weight loss following either Roux-en-Y gastric bypass surgery (RYGB) or an intensive lifestyle modification combined with best medical care (‘Lifestyle’)5.
| Baseline (pre-intervention) | Follow-up (7% wt loss) | RM-ANOVA | ||
|---|---|---|---|---|
| P (time) | P (time × intervention) | |||
| Total ATM (per g tissue) | ||||
| Lifestyle (n=7) | 54,772 (4,421 – 94,555) | 31,938 (19,849 – 45,750) | 0.850 | 0.320 |
| RYGB (n=6) | 30,042 (22,403 – 38,827) | 26,486 (13,672 – 58,831) | ||
| Total ATM (as % of CD45pos fraction) | ||||
| Lifestyle (n=7) | 12.8 (6.6 – 25.7) | 2.5 (1.9 – 5.6) | 0.004 | 0.511 |
| RYGB (n=6) | 11.4 (11.0 – 14.2) | 4.7 (1.7 – 8.2) | ||
| CD11cpos ATM (per g tissue) | ||||
| Lifestyle (n=7) | 5,502 (1,100 – 26,465) | 8,663 (5,833 – 9,798) | 0.793 | 0.209 |
| RYGB (n=6) | 7,163 (3,311 – 10,872) | 3,393 (1,701 – 4,922) | ||
| CD11cpos ATM (as % of CD45pos fraction) | ||||
| Lifestyle (n=7) | 3.6 (1.7 – 5.5) | 0.7 (0.6 – 0.8) | 0.010 | 0.596 |
| RYGB (n=6) | 2.8 (0.4 – 4.0) | 0.9 (0.6 – 1.3) | ||
| CD1cpos dendritic cells (per g tissue) | ||||
| Lifestyle (n=7) | 10,010 (2,455 – 22,830) | 9,582 (8,525 – 18,555) | 0.761 | 0.138 |
| RYGB (n=7) | 10,814 (9,009 – 21,054) | 9,934 (3,346 – 17,014) | ||
| CD1cpos dendritic cells (as % of CD45pos fraction) | ||||
| Lifestyle (n=5) | 3.1 (3.0 – 4.1) | 1.9 (1.4 – 4.1) | 0.003 | 0.440 |
| RYGB (n=6) | 3.7 (3.1 – 6.0) | 1.2 (1.0 – 1.6) | ||
| Neutrophil granulocytes (per g tissue) | ||||
| Lifestyle (n=7) | 6,192 (2,037 – 13,592) | 510,762 (337,602 – 695,103) | 0.001 | 0.049 |
| RYGB (n=7) | 14,913 (9,783 – 57,238) | 84,926 (16,049 – 849,753) | ||
| Neutrophil granulocytes (as % of CD45pos fraction) | ||||
| Lifestyle (n=7) | 4.0 (3.5 – 4.0) | 52.3 (42.7 – 64.0) | <0.001 | 0.090 |
| RYGB (n=7) | 4.8 (4.2 – 15.0) | 33.0 (17.2 – 64.5) | ||
| CD8pos T cells (per g tissue) | ||||
| Lifestyle (n=5) | 40,044 (24,564 – 56,744) | 56,812 (44,842 – 139,448) | 0.110 | 0.680 |
| RYGB (n=5) | 24,686 (22,635 – 25,677) | 35,897 (20,671 – 59,698) | ||
| CD8pos T cells (as % ofCD45pos fraction) | ||||
| Lifestyle (n=5) | 12.4 (11.5 – 23.8) | 9.3 (6.9 – 9.6) | 0.023 | 0.632 |
| RYGB (n=5) | 8.1 (7.2 – 9.5) | 7.1 (4.5 – 7.2) | ||
| CD4pos T cells (per g tissue) | ||||
| Lifestyle (n=5) | 61,091 (40,969 – 62,351) | 136,824 (67,707 – 171,496) | 0.041 | 0.408 |
| RYGB (n=5) | 53,817 (51,626 – 54,597) | 92,247 (61,368 – 121,248) | ||
| CD4pos T cells (as % of CD45pos fraction) | ||||
| Lifestyle (n=5) | 17.2 (17.1 – 19.3) | 14.6 (11.1 – 17.6) | 0.118 | 0.628 |
| RYGB (n=5) | 21.7 (19.9 – 22.7) | 11.5 (9.8 – 21.2) | ||
| CD4/CD8 T- cell ratio | ||||
| Lifestyle (n=5) | 0.97 (0.7 – 1.6) | 1.53 (1.2 – 3.0) | 0.005 | 0.040 |
| RYGB (n=5) | 2.21 (2.1 – 2.8) | 2.57 (2.2 – 3.0) | ||
4 Values are medians (25th, 75th percentiles). ATM: Adipose tissue macrophages; RM-ANOVA: Repeated measures analysis of variance.