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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2016 Feb 8;113(8):1647–1657. doi: 10.1002/bit.25939

Figure 4.

Figure 4

Analysis of selected clones from the Round 15 library. (A and B) Clones R15-25, 35 and 78 isolated from Round 15 all showed enhanced activity in assays when assayed in emulsion under selection-like conditions. To allow for better comparison, these assays were performed at a ratio of 100 genes per bead to eliminate the presence of any population of negative beads. Following emulsification, reactions were allowed to proceed overnight at 30°C after which the emulsion was broken and the beads were analyzed by flow cytometry without (A) or with additional signal amplification (B). (C) Analysis of selected clones without emulsification. Assays were performed as in (A) and (B) omitting the emulsification step. Analysis required amplification. (D) Flow cytometric analysis of purified clones. Beads bearing LPETG peptide were incubated in TBST with 5 uM GGG-FAM and 1.5 uM purified enzyme for 2 h at 37°C. Beads were then washed and analyzed by flow cytometry.