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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 17;101(1):152–166. doi: 10.1111/mmi.13381

Fig 1.

Fig 1

ITC analysis of B. japonicum Mur and E. coli Fur titrated with Mn2+ or Fe2+.

ITC raw data collection trace for titration of (A) Mn2+ to a sample of B. japonicum Mur, (B) Fe2+ to a sample of B. japonicum Mur, (C) Mn2+ to a sample of E. coli Fur, and (D) Fe2+ to a sample of E. coli Fur. (E) Dissociation binding constant (Kd) values and calculated number of sites per monomer (N) of B. japonicum Mur and E. coli Fur for Mn2+ and Fe2+ were determined using a one-binding-site model fitted to the titration data taken in triplicate.