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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 17;101(1):152–166. doi: 10.1111/mmi.13381

Fig 3.

Fig 3

Effect of metals on in vivo promoter occupancy and transcriptional repression by Mur in B. japonicum and E. coli cells.

Cells were grown in media with the presence or absence of 20 μM MnCl2 or 20 μM FeCl3. (A) In vivo occupancy of the mnoP promoter by Mur in B. japonicum cells (B) In vivo occupancy of the fiu promoter by Mur in E. coli cells. (C) In vivo occupancy of the mnoP promoter by Mur in E. coli cells. In vivo occupancy data are expressed as the relative starting quantity (SQ) of DNA normalized to the input, and are presented as the average of triplicate samples with the error bars representing the standard deviation. (D) Analysis of mnoP mRNA by qPCR in B. japonicum cells. (E) Analysis of fiu mRNA in E. coli cells. (F) Analysis of mnoP mRNA in E. coli cells. The data are expressed as the relative starting quantity (SQ) of mntH mRNA normalized to the housekeeping gene gapA, and are presented as the average of triplicate samples with the error bars representing the standard deviation.