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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Mol Microbiol. 2016 Apr 17;101(1):152–166. doi: 10.1111/mmi.13381

Fig. 5.

Fig. 5

Effects of high intracellular iron on in vivo promoter occupancy and transcriptional repression by Mur in B. japonicum cells

The intracellular iron concentration was elevated by growth in media containing 100 μM FeCl3 in the medium for B. japonicum, or by mutation of the iron exporter gene mbfA. Cells of the wild type and mutant were grown in media containing no added manganese and either 0, 20 μM or 100 μM FeCl3. (A) In vivo occupancy of the mnoP promoter by Mur in B. japonicum wild type (Wt) or mbfA strain. Data are expressed as the relative starting quantity (SQ) of DNA normalized to the input, and are presented as the average of triplicate samples with the error bars representing the standard deviation. (B) Analysis of mnoP mRNA by qPCR in B. japonicum wild type or mbfA strain. The data are expressed as the relative starting quantity (SQ) of mntH mRNA normalized to the housekeeping gene gapA, and are presented as the average of triplicate samples with the error bars representing the standard deviation.